Thymosin Beta-4 Reconstitution Calculator

What is Thymosin Beta-4?

Thymosin beta-4 is the full-length 43-amino-acid endogenous protein involved in cytoskeletal regulation through its binding of monomeric actin. Published research has studied thymosin beta-4 in models of cardiac repair, corneal wound healing, cutaneous wound healing, and inflammation modulation. It is distinct from the shorter TB-500 fragment also sold by research-chemical vendors. Thymosin beta-4 is not approved by the FDA for human use and is listed by WADA as a prohibited substance in sport. Research-grade thymosin beta-4 is labeled for laboratory research purposes only.

Common vial sizes

Research vendors typically offer thymosin beta-4 in 2 mg, 5 mg, or 10 mg lyophilized vials. Reconstitution volumes of 2–5 mL of bacteriostatic water are commonly reported.

• 2 mg vial
• 5 mg vial
• 10 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 1000 mcg dose = 40 units on a u-100 insulin, 1 ml (100 units) (0.4 mL).
• 5 mg vial + 3 mL bacteriostatic water → 1666.67 mcg/mL concentration.
  A 1500 mcg dose = 90 units on a u-100 insulin, 1 ml (100 units) (0.9 mL).
• 10 mg vial + 5 mL bacteriostatic water → 2000 mcg/mL concentration.
  A 2000 mcg dose = 100 units on a u-100 insulin, 1 ml (100 units) (1 mL).
• 2 mg vial + 2 mL bacteriostatic water → 1000 mcg/mL concentration.
  A 500 mcg dose = 50 units on a u-100 insulin, 1 ml (100 units) (0.5 mL).

Storage & shelf life

Lyophilized thymosin beta-4 is typically stored refrigerated or frozen. Reconstituted solutions are commonly refrigerated and protected from light; laboratory handling notes report stability on the order of weeks.

Reported half-life

Published work on thymosin beta-4 and its fragments reports a short plasma half-life for the parent peptide on the order of a few hours, with downstream cellular effects persisting longer.