Thymosin Beta-4 Calculator

Thymosin Beta-4 dosage calculator

The Thymosin Beta-4 dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. Thymosin Beta-4 is commonly sold in 2 mg, 5 mg, 10 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A 43-amino-acid actin-binding protein studied for its role in cytoskeletal regulation and cellular migration mechanisms. Enter your target dose in the calculator above to see the exact number of units to draw.

Thymosin Beta-4 dosage chart

Thymosin Beta-4 and Thymosin peptides

Thymosin Beta-4 is a Full-length 43-amino-acid thymosin beta-4. A 43-amino-acid actin-binding protein studied for its role in cytoskeletal regulation and cellular migration mechanisms. Other peptides in the Thymosin class include TB-500 .

Thymosin beta-4 is the full-length 43-amino-acid endogenous protein involved in cytoskeletal regulation through its binding of monomeric actin. Published research has studied thymosin beta-4 in models of cardiac repair, corneal wound healing, cutaneous wound healing, and inflammation modulation. It is distinct from the shorter TB-500 fragment also sold by research-chemical vendors. Thymosin beta-4 is not approved by the FDA for human use and is listed by WADA as a prohibited substance in sport. Research-grade thymosin beta-4 is labeled for laboratory research purposes only.

See all Thymosin peptides →

How to reconstitute Thymosin Beta-4

Research vendors typically offer thymosin beta-4 in 2 mg, 5 mg, or 10 mg lyophilized vials. Reconstitution volumes of 2–5 mL of bacteriostatic water are commonly reported.

Reconstituting Thymosin Beta-4 is the same unit-conversion process used for any lyophilized peptide:

1. Draw your chosen volume of bacteriostatic water into a syringe.
2. Inject the bacteriostatic water slowly down the side of the Thymosin Beta-4 vial — do not shoot it directly onto the powder.
3. Swirl gently until the powder fully dissolves. Do not shake.
4. Store the reconstituted vial refrigerated.
5. To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.

For Thymosin Beta-4 specifically, 2 mg, 5 mg, 10 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 2.5 mg/mL for the 5 mg vial size.

Thymosin Beta-4 half-life and storage

Published work on thymosin beta-4 and its fragments reports a short plasma half-life for the parent peptide on the order of a few hours, with downstream cellular effects persisting longer.

Lyophilized thymosin beta-4 is typically stored refrigerated or frozen. Reconstituted solutions are commonly refrigerated and protected from light; laboratory handling notes report stability on the order of weeks.

Common Thymosin Beta-4 dosing mistakes

Most Thymosin Beta-4 draw errors are unit errors, not arithmetic errors: confusing mg with mcg (1 mg = 1,000 mcg), or reading a U-40 syringe with U-100 math — the same printed mark holds 2.5× the volume. Two more worth avoiding: leaving the vial unlabeled, so the concentration and reconstitution date are lost, and under-diluting to the point where the draw is under about one unit and can no longer be read accurately.

Each of these is covered in full in the guides: reconstitution math from first principles, U-100 vs U-40 syringes, storage and shelf life, and how to reconstitute peptides.