Thymosin Beta-4 Calculator
Dosage and reconstitution math for Thymosin Beta-4. A 43-amino-acid actin-binding protein studied for its role in cytoskeletal regulation and cellular migration mechanisms.
Reconstitution calculator
Pre-filled with common defaults for Thymosin Beta-4. Adjust any field to match your own vial.
Show the math
Enter values above to see the step-by-step math.
Free Illustrated Reconstitution Guide (PDF)
8-step visual walkthrough with photos and tips — from gathering supplies to drawing your dose. Educational only — not medical advice.
Thymosin Beta-4 dosage calculator
The Thymosin Beta-4 dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. Thymosin Beta-4 is commonly sold in 2 mg, 5 mg, 10 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A 43-amino-acid actin-binding protein studied for its role in cytoskeletal regulation and cellular migration mechanisms. Enter your target dose in the calculator above to see the exact number of units to draw.
Thymosin Beta-4 dosage chart
| Sample dose | Concentration | Volume to draw | Units on U-100 |
|---|---|---|---|
| 0.13 mg | 1.66667 mg/mL | 0.078 mL | 7.8 |
| 0.25 mg | 1.66667 mg/mL | 0.15 mL | 15 |
| 0.5 mg | 1.66667 mg/mL | 0.3 mL | 30 |
| 1 mg | 1.66667 mg/mL | 0.6 mL | 60 |
| 2 mg | 1.66667 mg/mL | 1.2 mL | 120 |
Thymosin Beta-4 and Thymosin peptides
Thymosin Beta-4 is a Full-length 43-amino-acid thymosin beta-4. A 43-amino-acid actin-binding protein studied for its role in cytoskeletal regulation and cellular migration mechanisms. Other peptides in the Thymosin class include TB-500 .
How to reconstitute Thymosin Beta-4
Reconstituting Thymosin Beta-4 is the same unit-conversion process used for any lyophilized peptide:
- Draw your chosen volume of bacteriostatic water into a syringe.
- Inject the bacteriostatic water slowly down the side of the Thymosin Beta-4 vial — do not shoot it directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake.
- Store the reconstituted vial refrigerated.
- To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.
For Thymosin Beta-4 specifically, 2 mg, 5 mg, 10 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 2.5 mg/mL for the 5 mg vial size.
Thymosin Beta-4 half-life and storage
Published work on thymosin beta-4 and its fragments reports a short plasma half-life for the parent peptide on the order of a few hours, with downstream cellular effects persisting longer.
Lyophilized thymosin beta-4 is typically stored refrigerated or frozen. Reconstituted solutions are commonly refrigerated and protected from light; laboratory handling notes report stability on the order of weeks.
Common Thymosin Beta-4 dosing mistakes
- Confusing mg and mcg. 1 mg = 1,000 mcg. Always convert to the same unit before computing the draw.
- Using a U-40 syringe with U-100 math. Same printed mark, 2.5× the physical volume. Always check the syringe label.
- Not labeling the vial. Write the reconstitution date and concentration on the vial the moment you mix it.
- Under-diluting. If your draw is less than about 1 unit, the volume is too small to read accurately — add more bacteriostatic water next time.