DSIP Reconstitution Calculator

What is DSIP?

DSIP (delta sleep-inducing peptide) is a naturally occurring nine-amino-acid peptide first isolated from the cerebral venous blood of sleeping rabbits in the 1970s. Published research has studied DSIP in animal and limited human models of sleep regulation, stress response, and circadian biology, though the literature is older and mechanism remains incompletely characterized. DSIP is not approved by the FDA for human use. Research-grade DSIP sold by chemical supply companies is labeled for laboratory research only.

Common vial sizes

Research vendors typically offer DSIP in 2 mg or 5 mg lyophilized vials. Reconstitution with 2–3 mL of bacteriostatic water is commonly reported.

• 2 mg vial
• 5 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 100 mcg dose = 4 units on a u-100 insulin, 1 ml (100 units) (0.04 mL).
• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 250 mcg dose = 10 units on a u-100 insulin, 1 ml (100 units) (0.1 mL).
• 2 mg vial + 2 mL bacteriostatic water → 1000 mcg/mL concentration.
  A 100 mcg dose = 10 units on a u-100 insulin, 1 ml (100 units) (0.1 mL).
• 5 mg vial + 3 mL bacteriostatic water → 1666.67 mcg/mL concentration.
  A 500 mcg dose = 30 units on a u-100 insulin, 1 ml (100 units) (0.3 mL).

Storage & shelf life

Lyophilized DSIP is typically stored refrigerated or frozen. Reconstituted solutions are commonly refrigerated and protected from light.

Reported half-life

Published pharmacokinetic studies report a very short plasma half-life for the DSIP parent peptide, on the order of 7 minutes in some reports, with downstream physiological effects measured to persist beyond the circulating half-life.