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Copper-binding tripeptide

GHK-Cu Calculator

Dosage and reconstitution math for GHK-Cu. A copper-binding tripeptide isolated from human plasma, studied for its role in extracellular matrix signaling and wound healing.

Reconstitution calculator

Pre-filled with common defaults for GHK-Cu. Adjust any field to match your own vial.

mg
mL
Draw to
units on the syringe
Equivalent volume: mL
Concentration
mcg / mL
Per syringe unit
mcg / unit
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Enter values above to see the step-by-step math.

Preview of the illustrated reconstitution guide
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Free Illustrated Reconstitution Guide (PDF)

8-step visual walkthrough with photos and tips — from gathering supplies to drawing your dose. Educational only — not medical advice.

GHK-Cu reconstitution diagram GHK-Cu: 100 mg vial mixed with 5 mL bacteriostatic water yields 20 mg/mL. A 2 mg dose draws to 10 units on a U-100 syringe. 1 Reconstitute 100 mg vial + 5 mL BAC water 2 Calculate concentration 20 mg/mL 100 mg ÷ 5 mL = 20 concentration 3 Convert dose to volume 2 mg dose 2 mg ÷ 20 mg/mL = 0.1 mL 4 Draw to mark 0 25 50 75 100 10 units U-100 100 mg vial · 5 mL water · 20 mg/mL · 2 mg dose → 0.1 mL → 10 units on U-100

GHK-Cu dosage calculator

The GHK-Cu dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. GHK-Cu is commonly sold in 50 mg, 100 mg, 200 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A copper-binding tripeptide isolated from human plasma, studied for its role in extracellular matrix signaling and wound healing. Enter your target dose in the calculator above to see the exact number of units to draw.

GHK-Cu dosage chart

Sample doseConcentrationVolume to drawUnits on U-100
2.5 mg20 mg/mL0.125 mL12.5
5 mg20 mg/mL0.25 mL25
10 mg20 mg/mL0.5 mL50
20 mg20 mg/mL1 mL100
40 mg20 mg/mL2 mL200

GHK-Cu and Copper peptides

GHK-Cu is a Copper-binding tripeptide. A copper-binding tripeptide isolated from human plasma, studied for its role in extracellular matrix signaling and wound healing.

GHK-Cu is the copper complex of a naturally occurring human tripeptide (glycyl-L-histidyl-L-lysine) first isolated from human plasma. Published research has studied GHK and its copper complex for roles in wound healing, extracellular matrix remodeling, and anti-inflammatory signaling, with particular focus in cosmetic and dermatological contexts. It appears in a number of cosmetic formulations marketed topically. GHK-Cu sold by research chemical vendors in injectable lyophilized form is not approved by the FDA for injection and is labeled for laboratory research only.

See all Copper peptides →

How to reconstitute GHK-Cu

Research vendors typically offer GHK-Cu in larger vial sizes than most peptides — 50 mg, 100 mg, or 200 mg — because of the higher masses commonly used in published research. Reconstitution with 3–5 mL of bacteriostatic water is commonly reported.

Reconstituting GHK-Cu is the same unit-conversion process used for any lyophilized peptide:

  1. Draw your chosen volume of bacteriostatic water into a syringe.
  2. Inject the bacteriostatic water slowly down the side of the GHK-Cu vial — do not shoot it directly onto the powder.
  3. Swirl gently until the powder fully dissolves. Do not shake.
  4. Store the reconstituted vial refrigerated.
  5. To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.

For GHK-Cu specifically, 50 mg, 100 mg, 200 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 50 mg/mL for the 100 mg vial size.

GHK-Cu half-life and storage

Published pharmacokinetic data on injectable GHK-Cu is limited. As a small tripeptide complex, it is generally reported to have a plasma half-life on the order of hours.

Lyophilized GHK-Cu is typically stored refrigerated or frozen. Reconstituted solutions are generally refrigerated and protected from light. Copper complexes are especially sensitive to pH and should be stored according to supplier guidance.

Common GHK-Cu dosing mistakes

Most GHK-Cu draw errors are unit errors, not arithmetic errors: confusing mg with mcg (1 mg = 1,000 mcg), or reading a U-40 syringe with U-100 math — the same printed mark holds 2.5× the volume. Two more worth avoiding: leaving the vial unlabeled, so the concentration and reconstitution date are lost, and under-diluting to the point where the draw is under about one unit and can no longer be read accurately.

Each of these is covered in full in the guides: reconstitution math from first principles, U-100 vs U-40 syringes, storage and shelf life, and how to reconstitute peptides.

GHK-Cu FAQ

What is GHK-Cu? +
GHK-Cu is a Copper-binding tripeptide. A copper-binding tripeptide isolated from human plasma, studied for its role in extracellular matrix signaling and wound healing.
How do I calculate my GHK-Cu dose? +
Use the calculator above. Enter your vial size (50 mg, 100 mg, 200 mg are common for GHK-Cu), the amount of bacteriostatic water you added, and your target dose. The calculator returns the exact number of syringe units to draw.
What vial sizes does GHK-Cu come in? +
GHK-Cu is commonly available in 50 mg, 100 mg, 200 mg vials.
How long does GHK-Cu last? +
GHK-Cu's reported half-life is ~hours.
How should I store GHK-Cu? +
Lyophilized GHK-Cu is typically stored refrigerated or frozen. Reconstituted solutions are generally refrigerated and protected from light. Copper complexes are especially sensitive to pH and should be stored according to supplier guidance.

Published research on GHK-Cu

Indexed in PubMed. Listed for reference only — none of this is dosing guidance, and their presence here is not an endorsement of use.

General resources: PubMed, DailyMed, ClinicalTrials.gov.