Epithalon Reconstitution Calculator

What is Epithalon?

Epithalon, also spelled epitalon, is a synthetic four-amino-acid peptide (alanine-glutamic acid-aspartic acid-glycine) originally described by Russian researchers led by Vladimir Khavinson. It was designed as a short synthetic analog of the larger polypeptide epithalamin isolated from pineal gland tissue. Published research — much of it from Russian laboratories — has studied epithalon in cell and animal models in the contexts of telomerase activity, circadian regulation, and aging-related outcomes. Western peer-reviewed replication is more limited. Epithalon is not approved by the FDA for human use. Research-grade epithalon is labeled for laboratory research only.

Common vial sizes

Research vendors typically offer epithalon in 10 mg, 20 mg, 50 mg, or 100 mg lyophilized vials. Reconstitution with 2–5 mL of bacteriostatic water is commonly reported.

• 10 mg vial
• 20 mg vial
• 50 mg vial
• 100 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 50 mg vial + 3 mL bacteriostatic water → 16666.67 mcg/mL concentration.
  A 5000 mcg dose = 30 units on a u-100 insulin, 1 ml (100 units) (0.3 mL).
• 50 mg vial + 5 mL bacteriostatic water → 10000 mcg/mL concentration.
  A 10000 mcg dose = 100 units on a u-100 insulin, 1 ml (100 units) (1 mL).
• 100 mg vial + 5 mL bacteriostatic water → 20000 mcg/mL concentration.
  A 10000 mcg dose = 50 units on a u-100 insulin, 1 ml (100 units) (0.5 mL).
• 20 mg vial + 3 mL bacteriostatic water → 6666.67 mcg/mL concentration.
  A 5000 mcg dose = 75 units on a u-100 insulin, 1 ml (100 units) (0.75 mL).

Storage & shelf life

Lyophilized epithalon is typically stored refrigerated or frozen. Reconstituted solutions are commonly refrigerated and protected from light.

Reported half-life

As a very short unmodified tetrapeptide, epithalon is expected to have a short plasma half-life on the order of minutes, though downstream effects measured in research models may persist longer.