Thymosin Alpha-1 Reconstitution Calculator

What is Thymosin Alpha-1?

Thymosin alpha-1 is a 28-amino-acid peptide originally isolated from thymic tissue. Published research has studied its effects on immune-cell signaling, particularly T-cell maturation and cytokine modulation. Synthetic thymosin alpha-1 has been approved for clinical use in a number of countries outside the United States under the brand name Zadaxin for specific infectious and oncological indications, and has been the subject of clinical trials in viral hepatitis and immune dysfunction. It is not approved by the FDA for human use in the United States. Research-grade thymosin alpha-1 from chemical supply companies is labeled for laboratory research only.

Common vial sizes

Research vendors typically offer thymosin alpha-1 in 5 mg or 10 mg lyophilized vials. Reconstitution with 2–3 mL of bacteriostatic water is common.

• 5 mg vial
• 10 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 500 mcg dose = 20 units on a u-100 insulin, 1 ml (100 units) (0.2 mL).
• 5 mg vial + 3 mL bacteriostatic water → 1666.67 mcg/mL concentration.
  A 1000 mcg dose = 60 units on a u-100 insulin, 1 ml (100 units) (0.6 mL).
• 10 mg vial + 3 mL bacteriostatic water → 3333.33 mcg/mL concentration.
  A 1600 mcg dose = 48 units on a u-100 insulin, 1 ml (100 units) (0.48 mL).
• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 250 mcg dose = 10 units on a u-100 insulin, 1 ml (100 units) (0.1 mL).

Storage & shelf life

Lyophilized thymosin alpha-1 is typically stored refrigerated at 2–8 °C. Reconstituted solutions are generally reported to be kept refrigerated with light protection; stability is commonly reported in literature and manufacturer inserts.

Reported half-life

Published pharmacokinetic studies on synthetic thymosin alpha-1 report a plasma half-life of approximately 2 hours.