TB-500 Calculator

TB-500 dosage calculator

The TB-500 dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. TB-500 is commonly sold in 2 mg, 5 mg, 10 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A synthetic fragment of thymosin beta-4, the actin-sequestering protein studied for cytoskeletal dynamics in tissue repair. Enter your target dose in the calculator above to see the exact number of units to draw.

TB-500 dosage chart

TB-500 and Thymosin peptides

TB-500 is a Synthetic thymosin β-4 fragment. A synthetic fragment of thymosin beta-4, the actin-sequestering protein studied for cytoskeletal dynamics in tissue repair. Other peptides in the Thymosin class include Thymosin Beta-4 .

TB-500 is a synthetic peptide corresponding to an active region of thymosin beta-4, a naturally occurring 43-amino-acid protein implicated in cytoskeletal dynamics through its actin-binding activity. Published research has studied thymosin beta-4 and its fragments in models of cardiac, muscle, and cutaneous tissue repair, as well as inflammation modulation. The name "TB-500" refers specifically to the synthetic short-fragment version sold by research-chemical suppliers, which is distinct from full-length thymosin beta-4. TB-500 is not approved by the FDA for human use and has been banned by WADA as a prohibited substance in sport. Research-grade TB-500 is labeled for laboratory research only.

See all Thymosin peptides →

How to reconstitute TB-500

Research vendors typically offer TB-500 in 2 mg, 5 mg, and 10 mg lyophilized vials. Reconstitution with 2–5 mL of bacteriostatic water is common.

Reconstituting TB-500 is the same unit-conversion process used for any lyophilized peptide:

1. Draw your chosen volume of bacteriostatic water into a syringe.
2. Inject the bacteriostatic water slowly down the side of the TB-500 vial — do not shoot it directly onto the powder.
3. Swirl gently until the powder fully dissolves. Do not shake.
4. Store the reconstituted vial refrigerated.
5. To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.

For TB-500 specifically, 2 mg, 5 mg, 10 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 2.5 mg/mL for the 5 mg vial size.

TB-500 half-life and storage

Published work on synthetic thymosin beta-4 fragments reports a short plasma half-life on the order of a few hours for the parent peptide, while downstream cellular effects may persist longer.

Lyophilized TB-500 is commonly stored refrigerated or frozen. Reconstituted solutions are typically kept under refrigeration and protected from light; laboratory handling notes generally report stability on the order of weeks.

Common TB-500 dosing mistakes

Most TB-500 draw errors are unit errors, not arithmetic errors: confusing mg with mcg (1 mg = 1,000 mcg), or reading a U-40 syringe with U-100 math — the same printed mark holds 2.5× the volume. Two more worth avoiding: leaving the vial unlabeled, so the concentration and reconstitution date are lost, and under-diluting to the point where the draw is under about one unit and can no longer be read accurately.

Each of these is covered in full in the guides: reconstitution math from first principles, U-100 vs U-40 syringes, storage and shelf life, and how to reconstitute peptides.

TB-500 stacks

TB-500 is one of the ingredients in what is commonly referred to as the Wolverine stack . These are terms used in online peptide communities to describe specific combinations. See the stack pages for the full composition and calculator links.