TB-500 Calculator
Dosage and reconstitution math for TB-500. A synthetic fragment of thymosin beta-4, the actin-sequestering protein studied for cytoskeletal dynamics in tissue repair.
Reconstitution calculator
Pre-filled with common defaults for TB-500. Adjust any field to match your own vial.
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Enter values above to see the step-by-step math.
Free Illustrated Reconstitution Guide (PDF)
8-step visual walkthrough with photos and tips — from gathering supplies to drawing your dose. Educational only — not medical advice.
TB-500 dosage calculator
The TB-500 dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. TB-500 is commonly sold in 2 mg, 5 mg, 10 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A synthetic fragment of thymosin beta-4, the actin-sequestering protein studied for cytoskeletal dynamics in tissue repair. Enter your target dose in the calculator above to see the exact number of units to draw.
TB-500 dosage chart
| Sample dose | Concentration | Volume to draw | Units on U-100 |
|---|---|---|---|
| 0.13 mg | 1.66667 mg/mL | 0.078 mL | 7.8 |
| 0.25 mg | 1.66667 mg/mL | 0.15 mL | 15 |
| 0.5 mg | 1.66667 mg/mL | 0.3 mL | 30 |
| 1 mg | 1.66667 mg/mL | 0.6 mL | 60 |
| 2 mg | 1.66667 mg/mL | 1.2 mL | 120 |
TB-500 and Thymosin peptides
TB-500 is a Synthetic thymosin β-4 fragment. A synthetic fragment of thymosin beta-4, the actin-sequestering protein studied for cytoskeletal dynamics in tissue repair. Other peptides in the Thymosin class include Thymosin Beta-4 .
How to reconstitute TB-500
Reconstituting TB-500 is the same unit-conversion process used for any lyophilized peptide:
- Draw your chosen volume of bacteriostatic water into a syringe.
- Inject the bacteriostatic water slowly down the side of the TB-500 vial — do not shoot it directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake.
- Store the reconstituted vial refrigerated.
- To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.
For TB-500 specifically, 2 mg, 5 mg, 10 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 2.5 mg/mL for the 5 mg vial size.
TB-500 half-life and storage
Published work on synthetic thymosin beta-4 fragments reports a short plasma half-life on the order of a few hours for the parent peptide, while downstream cellular effects may persist longer.
Lyophilized TB-500 is commonly stored refrigerated or frozen. Reconstituted solutions are typically kept under refrigeration and protected from light; laboratory handling notes generally report stability on the order of weeks.
Common TB-500 dosing mistakes
- Confusing mg and mcg. 1 mg = 1,000 mcg. Always convert to the same unit before computing the draw.
- Using a U-40 syringe with U-100 math. Same printed mark, 2.5× the physical volume. Always check the syringe label.
- Not labeling the vial. Write the reconstitution date and concentration on the vial the moment you mix it.
- Under-diluting. If your draw is less than about 1 unit, the volume is too small to read accurately — add more bacteriostatic water next time.
TB-500 stacks
TB-500 is one of the ingredients in what is commonly referred to as the Wolverine stack . These are terms used in online peptide communities to describe specific combinations. See the stack pages for the full composition and calculator links.