TB-500 Reconstitution Calculator

What is TB-500?

TB-500 is a synthetic peptide corresponding to an active region of thymosin beta-4, a naturally occurring 43-amino-acid protein implicated in cytoskeletal dynamics through its actin-binding activity. Published research has studied thymosin beta-4 and its fragments in models of cardiac, muscle, and cutaneous tissue repair, as well as inflammation modulation. The name "TB-500" refers specifically to the synthetic short-fragment version sold by research-chemical suppliers, which is distinct from full-length thymosin beta-4. TB-500 is not approved by the FDA for human use and has been banned by WADA as a prohibited substance in sport. Research-grade TB-500 is labeled for laboratory research only.

Common vial sizes

Research vendors typically offer TB-500 in 2 mg, 5 mg, and 10 mg lyophilized vials. Reconstitution with 2–5 mL of bacteriostatic water is common.

• 2 mg vial
• 5 mg vial
• 10 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 1000 mcg dose = 40 units on a u-100 insulin, 1 ml (100 units) (0.4 mL).
• 5 mg vial + 3 mL bacteriostatic water → 1666.67 mcg/mL concentration.
  A 2000 mcg dose = 120 units on a u-100 insulin, 1 ml (100 units) (1.2 mL).
• 10 mg vial + 5 mL bacteriostatic water → 2000 mcg/mL concentration.
  A 2500 mcg dose = 125 units on a u-100 insulin, 1 ml (100 units) (1.25 mL).
• 2 mg vial + 2 mL bacteriostatic water → 1000 mcg/mL concentration.
  A 500 mcg dose = 50 units on a u-100 insulin, 1 ml (100 units) (0.5 mL).

Storage & shelf life

Lyophilized TB-500 is commonly stored refrigerated or frozen. Reconstituted solutions are typically kept under refrigeration and protected from light; laboratory handling notes generally report stability on the order of weeks.

Reported half-life

Published work on synthetic thymosin beta-4 fragments reports a short plasma half-life on the order of a few hours for the parent peptide, while downstream cellular effects may persist longer.