SS-31 (Elamipretide) Reconstitution Calculator

What is SS-31 (Elamipretide)?

SS-31, also known as elamipretide or MTP-131, is a synthetic mitochondrial-targeted tetrapeptide that has been studied for its binding to cardiolipin on the inner mitochondrial membrane. Published research has characterized its effects on electron transport chain function and reactive oxygen species in cellular and preclinical models, and it has been the subject of clinical trials in primary mitochondrial myopathy and cardiovascular disease contexts. As of this writing, SS-31 has not been approved by the FDA, though it has been investigated in multiple clinical programs. Research-grade SS-31 sold by chemical supply companies is labeled for laboratory use only.

Common vial sizes

Research vendors typically offer SS-31 in 5 mg, 10 mg, or 50 mg lyophilized vials. Reconstitution with 2–3 mL of bacteriostatic water is commonly reported.

• 5 mg vial
• 10 mg vial
• 50 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 10 mg vial + 2 mL bacteriostatic water → 5000 mcg/mL concentration.
  A 1000 mcg dose = 20 units on a u-100 insulin, 1 ml (100 units) (0.2 mL).
• 10 mg vial + 3 mL bacteriostatic water → 3333.33 mcg/mL concentration.
  A 2000 mcg dose = 60 units on a u-100 insulin, 1 ml (100 units) (0.6 mL).
• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 1000 mcg dose = 40 units on a u-100 insulin, 1 ml (100 units) (0.4 mL).
• 50 mg vial + 5 mL bacteriostatic water → 10000 mcg/mL concentration.
  A 5000 mcg dose = 50 units on a u-100 insulin, 1 ml (100 units) (0.5 mL).

Storage & shelf life

Lyophilized SS-31 is typically stored refrigerated. Reconstituted solutions are commonly refrigerated and protected from light.

Reported half-life

Published pharmacokinetic studies report a plasma half-life of approximately 3–4 hours for SS-31 / elamipretide.