SS-31 (Elamipretide) Calculator

SS-31 (Elamipretide) dosage calculator

The SS-31 (Elamipretide) dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. SS-31 (Elamipretide) is commonly sold in 5 mg, 10 mg, 50 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A mitochondrial-targeted tetrapeptide that binds cardiolipin on the inner mitochondrial membrane to modulate electron transport chain function. Enter your target dose in the calculator above to see the exact number of units to draw.

SS-31 (Elamipretide) dosage chart

SS-31 (Elamipretide) and Mitochondrial peptides

SS-31 (Elamipretide) is a Mitochondrial-targeted tetrapeptide. A mitochondrial-targeted tetrapeptide that binds cardiolipin on the inner mitochondrial membrane to modulate electron transport chain function. Other peptides in the Mitochondrial class include MOTS-c .

SS-31, also known as elamipretide or MTP-131, is a synthetic mitochondrial-targeted tetrapeptide that has been studied for its binding to cardiolipin on the inner mitochondrial membrane. Published research has characterized its effects on electron transport chain function and reactive oxygen species in cellular and preclinical models, and it has been the subject of clinical trials in primary mitochondrial myopathy and cardiovascular disease contexts. As of this writing, SS-31 has not been approved by the FDA, though it has been investigated in multiple clinical programs. Research-grade SS-31 sold by chemical supply companies is labeled for laboratory use only.

See all Mitochondrial peptides →

How to reconstitute SS-31 (Elamipretide)

Research vendors typically offer SS-31 in 5 mg, 10 mg, or 50 mg lyophilized vials. Reconstitution with 2–3 mL of bacteriostatic water is commonly reported.

Reconstituting SS-31 (Elamipretide) is the same unit-conversion process used for any lyophilized peptide:

1. Draw your chosen volume of bacteriostatic water into a syringe.
2. Inject the bacteriostatic water slowly down the side of the SS-31 (Elamipretide) vial — do not shoot it directly onto the powder.
3. Swirl gently until the powder fully dissolves. Do not shake.
4. Store the reconstituted vial refrigerated.
5. To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.

For SS-31 (Elamipretide) specifically, 5 mg, 10 mg, 50 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 5 mg/mL for the 10 mg vial size.

SS-31 (Elamipretide) half-life and storage

Published pharmacokinetic studies report a plasma half-life of approximately 3–4 hours for SS-31 / elamipretide.

Lyophilized SS-31 is typically stored refrigerated. Reconstituted solutions are commonly refrigerated and protected from light.

Common SS-31 (Elamipretide) dosing mistakes

Most SS-31 (Elamipretide) draw errors are unit errors, not arithmetic errors: confusing mg with mcg (1 mg = 1,000 mcg), or reading a U-40 syringe with U-100 math — the same printed mark holds 2.5× the volume. Two more worth avoiding: leaving the vial unlabeled, so the concentration and reconstitution date are lost, and under-diluting to the point where the draw is under about one unit and can no longer be read accurately.

Each of these is covered in full in the guides: reconstitution math from first principles, U-100 vs U-40 syringes, storage and shelf life, and how to reconstitute peptides.