NAD+ Reconstitution Calculator

What is NAD+?

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to cellular energy metabolism and redox biology. It is not a peptide but is commonly sold by research chemical vendors as an injectable research compound. Published research has studied NAD+ and its precursors (including nicotinamide riboside and nicotinamide mononucleotide) in the context of mitochondrial function, DNA repair pathways, and aging biology. Injectable NAD+ is not approved by the FDA for human use in the research-grade form sold by chemical supply companies. Research-grade NAD+ is labeled for laboratory use only.

Common vial sizes

Research vendors typically offer NAD+ in relatively large lyophilized vials — 100 mg, 500 mg, or 1000 mg — reflecting the higher masses associated with reported research protocols. Reconstitution with 3–10 mL of bacteriostatic water is commonly reported.

• 100 mg vial
• 500 mg vial
• 1000 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 500 mg vial + 5 mL bacteriostatic water → 100000 mcg/mL concentration.
  A 50000 mcg dose = 50 units on a u-100 insulin, 1 ml (100 units) (0.5 mL).
• 500 mg vial + 10 mL bacteriostatic water → 50000 mcg/mL concentration.
  A 100000 mcg dose = 200 units on a u-100 insulin, 1 ml (100 units) (2 mL).
• 1000 mg vial + 10 mL bacteriostatic water → 100000 mcg/mL concentration.
  A 100000 mcg dose = 100 units on a u-100 insulin, 1 ml (100 units) (1 mL).
• 100 mg vial + 3 mL bacteriostatic water → 33333.33 mcg/mL concentration.
  A 25000 mcg dose = 75 units on a u-100 insulin, 1 ml (100 units) (0.75 mL).

Storage & shelf life

Lyophilized NAD+ is typically stored refrigerated or frozen. Reconstituted solutions are commonly kept refrigerated and protected from light; NAD+ is known in the literature to be sensitive to hydrolysis and should be used promptly after reconstitution.

Reported half-life

Published pharmacokinetic data on injectable NAD+ is limited. Reports generally indicate a plasma half-life on the order of hours, though interpretation is complicated by rapid metabolism to downstream pyridines.