NAD+ Calculator
Dosage and reconstitution math for NAD+. A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.
Reconstitution calculator
Pre-filled with common defaults for NAD+. Adjust any field to match your own vial.
Show the math
Enter values above to see the step-by-step math.
Free Illustrated Reconstitution Guide (PDF)
8-step visual walkthrough with photos and tips — from gathering supplies to drawing your dose. Educational only — not medical advice.
NAD+ dosage calculator
The NAD+ dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. NAD+ is commonly sold in 100 mg, 500 mg, 1000 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways. Enter your target dose in the calculator above to see the exact number of units to draw.
NAD+ dosage chart
| Sample dose | Concentration | Volume to draw | Units on U-100 |
|---|---|---|---|
| 13 mg | 50 mg/mL | 0.26 mL | 26 |
| 25 mg | 50 mg/mL | 0.5 mL | 50 |
| 50 mg | 50 mg/mL | 1 mL | 100 |
| 100 mg | 50 mg/mL | 2 mL | 200 |
| 200 mg | 50 mg/mL | 4 mL | 400 |
NAD+ and Coenzyme peptides
NAD+ is a Coenzyme (nicotinamide adenine dinucleotide). A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to cellular energy metabolism and redox biology. It is not a peptide but is commonly sold by research chemical vendors as an injectable research compound. Published research has studied NAD+ and its precursors (including nicotinamide riboside and nicotinamide mononucleotide) in the context of mitochondrial function, DNA repair pathways, and aging biology. Injectable NAD+ is not approved by the FDA for human use in the research-grade form sold by chemical supply companies. Research-grade NAD+ is labeled for laboratory use only.
How to reconstitute NAD+
Research vendors typically offer NAD+ in relatively large lyophilized vials — 100 mg, 500 mg, or 1000 mg — reflecting the higher masses associated with reported research protocols. Reconstitution with 3–10 mL of bacteriostatic water is commonly reported.
Reconstituting NAD+ is the same unit-conversion process used for any lyophilized peptide:
- Draw your chosen volume of bacteriostatic water into a syringe.
- Inject the bacteriostatic water slowly down the side of the NAD+ vial — do not shoot it directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake.
- Store the reconstituted vial refrigerated.
- To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.
For NAD+ specifically, 100 mg, 500 mg, 1000 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 250 mg/mL for the 500 mg vial size.
NAD+ half-life and storage
Published pharmacokinetic data on injectable NAD+ is limited. Reports generally indicate a plasma half-life on the order of hours, though interpretation is complicated by rapid metabolism to downstream pyridines.
Lyophilized NAD+ is typically stored refrigerated or frozen. Reconstituted solutions are commonly kept refrigerated and protected from light; NAD+ is known in the literature to be sensitive to hydrolysis and should be used promptly after reconstitution.
Common NAD+ dosing mistakes
Most NAD+ draw errors are unit errors, not arithmetic errors: confusing mg with mcg (1 mg = 1,000 mcg), or reading a U-40 syringe with U-100 math — the same printed mark holds 2.5× the volume. Two more worth avoiding: leaving the vial unlabeled, so the concentration and reconstitution date are lost, and under-diluting to the point where the draw is under about one unit and can no longer be read accurately.
Each of these is covered in full in the guides: reconstitution math from first principles, U-100 vs U-40 syringes, storage and shelf life, and how to reconstitute peptides.
NAD+ stacks
NAD+ is one of the ingredients in what is commonly referred to as the Longevity stack . These are terms used in online peptide communities to describe specific combinations. See the stack pages for the full composition and calculator links.
NAD+ FAQ
What is NAD+? +
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Related peptides
Published research on NAD+
Indexed in PubMed. Listed for reference only — none of this is dosing guidance, and their presence here is not an endorsement of use.
- NAD(+) metabolism and its roles in cellular processes during ageing Nat Rev Mol Cell Biol, 2021 · PMID 33353981
- NAD+ and sirtuins in aging and disease Trends Cell Biol, 2014 · PMID 24786309
- NAD(+) metabolism, stemness, the immune response, and cancer Signal Transduct Target Ther, 2021 · PMID 33384409
- NAD metabolism: Role in senescence regulation and aging Aging Cell, 2024 · PMID 37424179
General resources: PubMed, DailyMed, ClinicalTrials.gov.