NAD+ Calculator
Dosage and reconstitution math for NAD+. A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.
Reconstitution calculator
Pre-filled with common defaults for NAD+. Adjust any field to match your own vial.
Show the math
Enter values above to see the step-by-step math.
Free Illustrated Reconstitution Guide (PDF)
8-step visual walkthrough with photos and tips — from gathering supplies to drawing your dose. Educational only — not medical advice.
NAD+ dosage calculator
The NAD+ dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. NAD+ is commonly sold in 100 mg, 500 mg, 1000 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways. Enter your target dose in the calculator above to see the exact number of units to draw.
NAD+ dosage chart
| Sample dose | Concentration | Volume to draw | Units on U-100 |
|---|---|---|---|
| 13 mg | 50 mg/mL | 0.26 mL | 26 |
| 25 mg | 50 mg/mL | 0.5 mL | 50 |
| 50 mg | 50 mg/mL | 1 mL | 100 |
| 100 mg | 50 mg/mL | 2 mL | 200 |
| 200 mg | 50 mg/mL | 4 mL | 400 |
NAD+ and Coenzyme peptides
NAD+ is a Coenzyme (nicotinamide adenine dinucleotide). A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.
How to reconstitute NAD+
Reconstituting NAD+ is the same unit-conversion process used for any lyophilized peptide:
- Draw your chosen volume of bacteriostatic water into a syringe.
- Inject the bacteriostatic water slowly down the side of the NAD+ vial — do not shoot it directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake.
- Store the reconstituted vial refrigerated.
- To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.
For NAD+ specifically, 100 mg, 500 mg, 1000 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 250 mg/mL for the 500 mg vial size.
NAD+ half-life and storage
Published pharmacokinetic data on injectable NAD+ is limited. Reports generally indicate a plasma half-life on the order of hours, though interpretation is complicated by rapid metabolism to downstream pyridines.
Lyophilized NAD+ is typically stored refrigerated or frozen. Reconstituted solutions are commonly kept refrigerated and protected from light; NAD+ is known in the literature to be sensitive to hydrolysis and should be used promptly after reconstitution.
Common NAD+ dosing mistakes
- Confusing mg and mcg. 1 mg = 1,000 mcg. Always convert to the same unit before computing the draw.
- Using a U-40 syringe with U-100 math. Same printed mark, 2.5× the physical volume. Always check the syringe label.
- Not labeling the vial. Write the reconstitution date and concentration on the vial the moment you mix it.
- Under-diluting. If your draw is less than about 1 unit, the volume is too small to read accurately — add more bacteriostatic water next time.
NAD+ stacks
NAD+ is one of the ingredients in what is commonly referred to as the Longevity stack . These are terms used in online peptide communities to describe specific combinations. See the stack pages for the full composition and calculator links.