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Coenzyme (nicotinamide adenine dinucleotide)

NAD+ Calculator

Dosage and reconstitution math for NAD+. A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.

Reconstitution calculator

Pre-filled with common defaults for NAD+. Adjust any field to match your own vial.

mg
mL
Draw to
units on the syringe
Equivalent volume: mL
Concentration
mcg / mL
Per syringe unit
mcg / unit
Show the math

Enter values above to see the step-by-step math.

Preview of the illustrated reconstitution guide
Free download

Free Illustrated Reconstitution Guide (PDF)

8-step visual walkthrough with photos and tips — from gathering supplies to drawing your dose. Educational only — not medical advice.

NAD+ reconstitution diagram NAD+: 500 mg vial mixed with 10 mL bacteriostatic water yields 50 mg/mL. A 50 mg dose draws to 100 units on a U-100 syringe. 1 Reconstitute 500 mg vial + 10 mL BAC water 2 Calculate concentration 50 mg/mL 500 mg ÷ 10 mL = 50 concentration 3 Convert dose to volume 50 mg dose 50 mg ÷ 50 mg/mL = 1 mL 4 Draw to mark 0 25 50 75 100 100 units U-100 500 mg vial · 10 mL water · 50 mg/mL · 50 mg dose → 1 mL → 100 units on U-100

NAD+ dosage calculator

The NAD+ dosage calculator above converts a target dose into exact syringe units based on your vial size and how much bacteriostatic water you used to reconstitute it. NAD+ is commonly sold in 100 mg, 500 mg, 1000 mg vials. The calculator supports both U-100 and U-40 insulin syringes and flags draws that are too large for the syringe barrel or too small to measure accurately. A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways. Enter your target dose in the calculator above to see the exact number of units to draw.

NAD+ dosage chart

Sample doseConcentrationVolume to drawUnits on U-100
13 mg50 mg/mL0.26 mL26
25 mg50 mg/mL0.5 mL50
50 mg50 mg/mL1 mL100
100 mg50 mg/mL2 mL200
200 mg50 mg/mL4 mL400

NAD+ and Coenzyme peptides

NAD+ is a Coenzyme (nicotinamide adenine dinucleotide). A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to cellular energy metabolism and redox biology. It is not a peptide but is commonly sold by research chemical vendors as an injectable research compound. Published research has studied NAD+ and its precursors (including nicotinamide riboside and nicotinamide mononucleotide) in the context of mitochondrial function, DNA repair pathways, and aging biology. Injectable NAD+ is not approved by the FDA for human use in the research-grade form sold by chemical supply companies. Research-grade NAD+ is labeled for laboratory use only.

See all Coenzyme peptides →

How to reconstitute NAD+

Research vendors typically offer NAD+ in relatively large lyophilized vials — 100 mg, 500 mg, or 1000 mg — reflecting the higher masses associated with reported research protocols. Reconstitution with 3–10 mL of bacteriostatic water is commonly reported.

Reconstituting NAD+ is the same unit-conversion process used for any lyophilized peptide:

  1. Draw your chosen volume of bacteriostatic water into a syringe.
  2. Inject the bacteriostatic water slowly down the side of the NAD+ vial — do not shoot it directly onto the powder.
  3. Swirl gently until the powder fully dissolves. Do not shake.
  4. Store the reconstituted vial refrigerated.
  5. To dose, calculate your draw volume: divide your target dose by the concentration (vial mg ÷ water mL = mg/mL). The calculator above does this automatically.

For NAD+ specifically, 100 mg, 500 mg, 1000 mg vials are common. A typical reconstitution adds 2 mL of bacteriostatic water, producing a concentration of 250 mg/mL for the 500 mg vial size.

NAD+ half-life and storage

Published pharmacokinetic data on injectable NAD+ is limited. Reports generally indicate a plasma half-life on the order of hours, though interpretation is complicated by rapid metabolism to downstream pyridines.

Lyophilized NAD+ is typically stored refrigerated or frozen. Reconstituted solutions are commonly kept refrigerated and protected from light; NAD+ is known in the literature to be sensitive to hydrolysis and should be used promptly after reconstitution.

Common NAD+ dosing mistakes

Most NAD+ draw errors are unit errors, not arithmetic errors: confusing mg with mcg (1 mg = 1,000 mcg), or reading a U-40 syringe with U-100 math — the same printed mark holds 2.5× the volume. Two more worth avoiding: leaving the vial unlabeled, so the concentration and reconstitution date are lost, and under-diluting to the point where the draw is under about one unit and can no longer be read accurately.

Each of these is covered in full in the guides: reconstitution math from first principles, U-100 vs U-40 syringes, storage and shelf life, and how to reconstitute peptides.

NAD+ stacks

NAD+ is one of the ingredients in what is commonly referred to as the Longevity stack . These are terms used in online peptide communities to describe specific combinations. See the stack pages for the full composition and calculator links.

NAD+ FAQ

What is NAD+? +
NAD+ is a Coenzyme (nicotinamide adenine dinucleotide). A coenzyme central to cellular energy metabolism, redox biology, and NAD+-dependent enzymatic pathways.
How do I calculate my NAD+ dose? +
Use the calculator above. Enter your vial size (100 mg, 500 mg, 1000 mg are common for NAD+), the amount of bacteriostatic water you added, and your target dose. The calculator returns the exact number of syringe units to draw.
What vial sizes does NAD+ come in? +
NAD+ is commonly available in 100 mg, 500 mg, 1000 mg vials.
How long does NAD+ last? +
NAD+'s reported half-life is ~hours.
How should I store NAD+? +
Lyophilized NAD+ is typically stored refrigerated or frozen. Reconstituted solutions are commonly kept refrigerated and protected from light; NAD+ is known in the literature to be sensitive to hydrolysis and should be used promptly after reconstitution.

Published research on NAD+

Indexed in PubMed. Listed for reference only — none of this is dosing guidance, and their presence here is not an endorsement of use.

General resources: PubMed, DailyMed, ClinicalTrials.gov.