MOTS-c Reconstitution Calculator

What is MOTS-c?

MOTS-c (Mitochondrial Open Reading frame of the Twelve S rRNA type-c) is a 16-amino-acid peptide encoded by a short open reading frame within mitochondrial DNA. Published research has characterized MOTS-c as a mitochondrial-derived peptide that has been studied for its roles in metabolic homeostasis, glucose regulation, and AMPK-related signaling in cell and animal models. The published literature on MOTS-c is expanding but still primarily preclinical. MOTS-c is not approved by the FDA for human use. Research-grade MOTS-c is sold as a laboratory reagent only.

Common vial sizes

Research vendors typically offer MOTS-c in 5 mg or 10 mg lyophilized vials. Reconstitution with 2–3 mL of bacteriostatic water is commonly reported.

• 5 mg vial
• 10 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 10 mg vial + 2 mL bacteriostatic water → 5000 mcg/mL concentration.
  A 1000 mcg dose = 20 units on a u-100 insulin, 1 ml (100 units) (0.2 mL).
• 10 mg vial + 3 mL bacteriostatic water → 3333.33 mcg/mL concentration.
  A 2000 mcg dose = 60 units on a u-100 insulin, 1 ml (100 units) (0.6 mL).
• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 1000 mcg dose = 40 units on a u-100 insulin, 1 ml (100 units) (0.4 mL).
• 10 mg vial + 3 mL bacteriostatic water → 3333.33 mcg/mL concentration.
  A 5000 mcg dose = 150 units on a u-100 insulin, 1 ml (100 units) (1.5 mL).

Storage & shelf life

Lyophilized MOTS-c is typically stored refrigerated or frozen. Reconstituted solutions are commonly refrigerated and protected from light.

Reported half-life

Published pharmacokinetic data on MOTS-c is limited. As a mid-sized peptide without stability modifications, it is generally reported to have a plasma half-life on the order of a few hours.