Semaglutide Reconstitution Calculator

What is Semaglutide?

Semaglutide is a synthetic analog of glucagon-like peptide-1 (GLP-1), an incretin hormone secreted by intestinal L-cells in response to nutrient intake. The molecule binds the GLP-1 receptor and has been studied extensively in published research for its effects on glucose-dependent insulin secretion, gastric emptying, and central appetite signaling. Structural modifications — notably a fatty acid side chain that reversibly binds albumin — extend its plasma half-life substantially compared to native GLP-1. Semaglutide has been the subject of large clinical trials reported in journals including the New England Journal of Medicine, and has been approved by regulatory agencies under brand names including Ozempic, Rybelsus, and Wegovy for use by prescription. Research-grade semaglutide sold by chemical supply companies is labeled for laboratory use only and is not approved by the FDA for human consumption in that form.

Common vial sizes

Research chemical vendors typically offer semaglutide in lyophilized vials of 2 mg, 3 mg, 5 mg, and 10 mg. A 5 mg vial reconstituted with 1 mL of bacteriostatic water yields a 5,000 mcg/mL solution.

• 2 mg vial
• 3 mg vial
• 5 mg vial
• 10 mg vial

Reconstitution examples

Worked examples showing exactly what a given vial, bacteriostatic water volume, and target dose produce in syringe units. All math is from the same calculator above.

• 5 mg vial + 1 mL bacteriostatic water → 5000 mcg/mL concentration.
  A 250 mcg dose = 5 units on a u-100 insulin, 1 ml (100 units) (0.05 mL).
• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 250 mcg dose = 10 units on a u-100 insulin, 1 ml (100 units) (0.1 mL).
• 5 mg vial + 2 mL bacteriostatic water → 2500 mcg/mL concentration.
  A 500 mcg dose = 20 units on a u-100 insulin, 1 ml (100 units) (0.2 mL).
• 10 mg vial + 2 mL bacteriostatic water → 5000 mcg/mL concentration.
  A 1000 mcg dose = 20 units on a u-100 insulin, 1 ml (100 units) (0.2 mL).

Storage & shelf life

Lyophilized semaglutide is typically stored refrigerated at 2–8 °C and protected from light. Reconstituted solutions are generally reported in manufacturer literature to remain stable for several weeks under refrigeration, with exact figures varying by buffer and vial conditions.

Reported half-life

Published pharmacokinetic studies report a terminal half-life of approximately 7 days in humans, reflecting albumin binding and slow receptor-mediated clearance.